Funny how they sometimes teach you the 'wrong' ones in school. I think the obvious pick is 'Murder on the Orient Express' – it's the one everyone knows, and it's absolutely deserved. The whole setup, the claustrophobic train, that impossible crime, and then Poirot's solution... it's just iconic in a way few mysteries ever are. But it's also a bit of a cheat because the real bestseller might be 'And Then There Were None,' even though he's not in it! Still, for Poirot, 'Orient Express' feels like his magnum opus.
That said, my personal favorite is 'The Murder of Roger Ackroyd.' The structure of that book, the way the narration works, it genuinely changed how I thought about mystery novels. It was a huge seller and caused a huge stir back in the day. 'Orient Express' might be the crowd-pleaser, but 'Ackroyd' is the one that messed with the rules. It's the one I go back to.